MEDICAMENTOS ANTIEPILÉPTICOS CAUSAM EFEITOS LETAIS, SUBLETAIS, TERATOGÊNICOS E PARÂMETROS MORFOMÉTRICOS EM EMBRIÕES E LARVAS DE ZEBRAFISH (DANIO RERIO)

doi.org/10.51891/rease.v6i12.663

Abstract

: In the present study, we evaluated the toxic effects of antiepileptic drugs gabapentin (GAB), phenobarbital (PB), oxcarbazepine (OX), and lamotrigine (LTG) the lethal, sublethal, teratogenic effects, and morphometric parameters on embryos and larvae of zebrafish (Danio rerio). An acute toxicity test was performed using D. rerio embryos according to OECD 236 (2013) guidelines. The acute toxicity test revealed that the mortality of animals increased among the time (24 to 96 hpf) on animals exposed to GAB, PB, OX, and LTG. The sublethal effects had a significant (p < 0.05) variation in the number of heartbeats, not a dose-dependent manner. It was also possible to observe variation in the pigmentation in all animals and the presence of pericardial edema in some groups. The teratogenic effects revealed the malformation of the head only in the animals exposed to GAB. In addition, tail deformation, spine deformation, yolk sac edema, and inflated swim bladder were observed in all animals. The morphometric parameters had a decrease (p <0.05) in body length, head-width, ocular distance, the distance between eyes, and eye diameter in all groups in the 96 hpf. It has been observed that gabapentin, phenobarbital, oxcarbazepine, and lamotrigine induce lethal, sublethal, teratogenic effects, and morphometric alterations on zebrafish.